Abstract:

The geographic origin of wines was discriminated by determining C, H and O isotopes using site-specific natural
isotopic fractionation nuclear magnetic resonance (SNIF-NMR) and isotope ratio mass spectrometry (IRMS). Sixty samples
of wine from four different regions including Changli, Yantai, Helanshan and Shacheng produced during the years 2010 to
2012 were collected. The values of (D/H)i, (D/H)ii, R, δ13C, δ18O and alcohol content (V/V) of these samples were 95.68 ×
10-6–99.15 × 10-6, 115.99 × 10-6–126.39 × 10-6, 2.38–2.59, −28.35‰–−23.00‰, −1.94‰–−0.05‰ and 11.3%–12.9%,
respectively. Generally, all the data were widely distributed but centralized for the same region. It was found that one isotope
could only be used to discriminate wines from growing regions with significantly different climatic conditions. By taking all
three isotopes into account, four wine-producing regions could be distinguished effectively with 100% accuracy. Based on
isotope data and alcohol content of wines from Shacheng, the adulterated wines could be distinguished by t-test. Among five
unknown wines tested, only one was found to be an authentic wine made from Shacheng grape.

Key words: food quality, site-specific natural isotopic fractionation-nuclear magnetic resonance (SNIF-NMR), isotope ratio mass spectrometry (IRMS), linear discriminant analysis, stable isotope