Isolation and Identification of Anthocyanins from Solanum nigrum L. Fruits

doi:10.7506/spkx1002-6630-201607011

Abstract

Abstract:

The objective of this study was to isolate anthocyanins from Solanum nigrum L. fruits by silica gel column
chromatography and identify them by UV-visible spectroscopy and high performance liquid chromatography-electrospray
ionization tandem mass spectrometry (HPLC-DAD-ESI-MS/MS). Acid hydrolysis was applied for the identification
of glucosides. Three anthocyanins were identified for the first time in Solanum nigrum L. fruits. Only delphinidin-3-
succinylarabinoside was detected in fraction I (the purity was 94%), while both cyanidin-3-galactoside (the purity was
45.67%) and cyanidin-3-acetylgalactoside (the purity was 13.97%) were found to be present in fraction II. The presented
method is simple and reliable to isolate and identify the anthocyanins from Solanum nigrum L. fruits.

Key words: Solanum nigrum L., anthocyanins, isolation, identification, high performance liquid chromatography electrosprary ionization-mass spectrometry (HPLC-DAD-ESI-MS/MS), acid hydrolysis

CLC Number:

TS201.1

TENG Fei, ZHENG Yue, WANG Ping. Isolation and Identification of Anthocyanins from Solanum nigrum L. Fruits[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201607011.

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Isolation and Identification of Anthocyanins from Solanum nigrum L. Fruits

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