Abstract:

A reversed-phase high performance liquid chromatographic (RP-HPLC) method has been presented for the
simultaneous determination of 17 free amino acids and 7 biogenic amines in Chinese rice wine. Wine samples were precolumn
derivatized with phenylisothiocyanate (PITC), separated using Inertsil ODS-SP C18 chromatography column (250 mm ×
4.6 mm, 5 μm), eluted with a mobile phase composed of sodium acetate, acetonitrile and ultra-pure water, and detected at
254 nm. The calibration curves of 17 amino acids and 7 biogenic amines all exhibited good linearity over the range from 2.0
to 500 mg/L and 0.5 to 125 mg/L, respectively. Mean recoveries for amino acids and biogenic amines were between 86.44%
and 99.75%, and the relative standard deviations (RSDs) were in the range of 0.31%–3.53%. The limits of detection (LODs)
for 17 free amino acids and 7 biogenic amines were in the range of 0.02–0.25 mg/L, and the limits of quantification (LOQs)
were 0.25–4.50 mg/L. The method is stable, accurate, and suitable for the simultaneous determination of amino acids and
biogenic amines in Chinese rice wine, having a wide linear range.

Key words: Chinese wice wine, amino acids, biogenic amines, reversed-phase high performance liquid chromatography (RP-HPLC)