Abstract:

A high performance liquid chromatography (HPLC) method was developed in this study for the simultaneous
determination of 16 additives in wine. Chromatographic separation was performed using gradient elution on CAPCELL PAK
C18 column (150 mm × 4.6 mm, 5 μm) with methanol-20 mmol/L ammonium acetate as mobile phase, followed by detection at
250 nm. The results showed that the method exhibited an excellent linearity in the concentration range of 0.1–20 mg/L with a
correlation coefficient of 0.999 3–0.999 7 and had a good repeatability with relative standard deviation (RSD) of 0.3%–6.3%. The
limits of detection (LOD) were between 0.01 and 0.5 mg/L, and average recoveries ranged from 76.5% to 108.5%. The present
method is simple and accurate and can meet the requirements for the analysis of illicit additives in wines.

Key words: high performance liquid chromatography (HPLC), additives, wine