Abstract:

Flavonoids from the dried fruits of Choerospondias axillaris were separated using preparative chromatography
into two fractions (F1 and F2). The total antioxidant capacity, and DPPH radical, hydroxyl radical and superoxide anion
radical scavenging activities of F1 and F2 were evaluated using ascorbic acid (VC) as the reference. The results demonstrated
that total flavonoids from C. axillaris had obvious antioxidant capacity, which was between F1 and F2. The DPPH radical
scavenging activity of F1 and the hydroxyl radical scavenging activities of F1 and F2 were higher than those of VC, but total
antioxidant capacity and superoxide anion radical scavenging activity of F1 and F2 were lower than those of VC. In addition,
as evaluated using cell model, the flavonoids from C. axillaris showed significant antioxidant activity in mouse macrophages
(RAW264.7).

Key words: flavonoids from Choerospondias axillaris fruits, total antioxidant capacity, free radical scavenging activity, cellular antioxidant activity, mouse macrophages RAW264.7