Purification and Bioactivity Evaluation of Proanthocyanidins from Spatholobi Caulis

doi:10.7506/spkx1002-6630-201617008

Abstract

Abstract:

Proanthocyanidins from Spatholobi Caulis were purified by macroporous adsorption resin, and then their purity,
1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity and α-glucosidase inhibitory activity were evaluated.
In terms of static adsorption and desorption capacity, X-5 resin was chosen as the better sorbent for the purification of
proanthocyanidins compared with D101 and AB-8 resin. Afterwards, the optimal parameters of dynamic adsorption and
desorption for proanthocyanidin purification were obtained as follows: sample concentration, 6.00 mg/mL; loading rate,
2 BV/h; loading amount, 10 BV; eluent flow rate, 1 BV/h; and eluent volume, 2 BV. Proanthocyanidins with different purities were
obtained by elution with different concentrations of ethanol, and the use of 70% ethanol as eluent gave the highest purity and strongest
DPPH radical scavenging activity as well as α-glucosidase inhibitory activity. The correlation analysis indicated that proanthocyanidins
might be the major components responsible for the antioxidant activity and α-glucosidase inhibitory activity of Spatholobi Caulis.

Key words: Spatholobi Caulis, proanthocyanidins, macroporous adsorption resin purification, 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity, α-glucosidase inhibitory activity

CLC Number:

TS209

LI Yan, LI Xin, LIU Jingling, ZHANG Chenlu, LIANG Zongsuo. Purification and Bioactivity Evaluation of Proanthocyanidins from Spatholobi Caulis[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201617008.

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Purification and Bioactivity Evaluation of Proanthocyanidins from Spatholobi Caulis

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