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Fast Determination of Phenolics and Polyphenolics in Wine by Ultra Performance Liquid Chromatography

ZHANG Xingxing1, GUO Anque1,2, HAN Fuliang1,2, ZHANG Yulin1,2,*   

  1. 1. College of Enology, Northwest A&F University, Yangling 712100, China;
    2. Shaanxi Engineering Research Center for Viti-Viniculture, Yangling 712100, China
  • Online:2016-05-25 Published:2016-05-18

Abstract:

The aim of study was to develop a novel method using ultra-performance liquid chromatography (UPLC) to rapidly
determine monophenols in wine. The UPLC system equipped with C18 BEH analytical column (2.1 mm × 50 mm, 1.7 μm)
was kept at 30 ℃ by column oven, utilizing A 1% acetic acid and B acetonitrile mobile phase in the gradient elution mode
within 15 min. The flow rate was 0.2 mL/min. A diode array detector (DAD) was used to monitor signals at 210?400 nm.
The method precision (n = 6) for 17 compounds expressed as RSD was 0.25%?0.66% and average recovery rates of spiked
wine samples were 85.10%?102.33%. The new method was simple, time-saving, accurate, reliable and prefect reproducible.
Therefore, it is a promising method for the identification and quantitation of phenolic compounds in wine.

Key words: ultra performance liquid chromatography (UPLC), wine, monophenols, assay

CLC Number: