Cloning and Expression of Mannitol Dehydrogenase Gene from Leuconostoc pseudomesenteroides

doi:10.7506/spkx1002-6630-201613027

Abstract

Abstract:

The structural gene encoding mannitol dehydrogenase (MDH) from Leuconostoc pseudomesenteroides was
amplified by PCR and cloned into the vector pETDuet-1. As a result, the plasmid pETDuet-1-mdh was constructed and
transformed into E. coli BL21(DE3) for MDH expression induced by isopropyl-β-D-thiogalactoside (IPTG). The length
of the mdh structural gene was l 017 bp. The recombinant mdh gene was successfully expressed in Escherichia coli and
the molecular weight of the expressed protein was 36.0 kD. The activity of recombinant mannitol dehydrogenase was
0.15 U/mg pro, which was higher than the MDH activity of Leuconostoc pseudomesenteroides (0.03 U/mg pro).

Key words: mannitol, mannitol dehydrogenase, Leuconostoc pseudomesenteroides, gene cloning, expression

CLC Number:

TS201.3

CHENG Yayun, WANG Xin, ZHENG Lin, LI Guanhao, CUI Hushan, JIN Qing. Cloning and Expression of Mannitol Dehydrogenase Gene from Leuconostoc pseudomesenteroides[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201613027.

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