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Isolation and Identification of Lactobacillus salivarius from Chicken Intestine and Its Biological Characteristics

CHEN Xinliang, SHAO Qibing, WANG Chao, HE Jianbin, ZHANG Yi, YANG Shuhua, LI Peng, LONG Miao   

  1. College of Animal Husbandry and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, China
  • Online:2016-07-15 Published:2016-07-26
  • Contact: LONG Miao

Abstract:

An isolate of the anaerobic bacterium Lactobacillus salivarius was obtained from the intestine of chicken and
identified by colony morphology, Gram staining, physio-biochemical reactions, and 16S rRNA sequencing analysis. Results
showed that the strain was Lactobacillus salivarius and named as Lactobacillus salivarius C-1-3. Then the strain was
subjected to antibacterial, cellulose degradation, and acid, bile salt and high temperature tolerance tests. It turned out that
the strain C-1-3 had a powerful antibacterial effect against E. coli, Salmonella and Staphylococcus aureus. And it also had
cellulose degradation ability. After several generations of domestication, this strain possessed good antibacterial performance
and significant tolerance to acid and high temperature.

Key words: Lactobacillus salivarius, isolation and identification, biological characteristics

CLC Number: