Enrichment and Isolation of Salmonella enteritidis by Immune Magnetic Nanoparticles

doi:10.7506/spkx1002-6630-201613029

Abstract

Abstract:

In this study, carboxyl-coated magnetic namoparticles (MNPs) were prepared using a chemical coprecipitation
method. The coupling conditions for conjugates (immune MNPs) of MNPs and anti-Salmonella polyclonal antibody (PAb)
were optimized, and then the application of immune MNPs in the enrichment and isolation of Salmonella enteritidis was
evaluated, which will provide a more fast and effective way for the detection of Salmonella. The results showed that the
optimal coupling of MNPs and PAb was achieved when the volume ratio of 51.7 μg/mL MNPs to 0.4 mol/L EDC-0.1 mol/L
NHS to 1.0 mg/mL PAb was 1:2:2 in a water bath at 37 ℃ for 40 min. The adsorption efficiency attained 94.36% when the volume
ratio between 51.7 μg/mL immune MNPs and 104 CFU/mL S. enteritidis was 4:5, and the incubation time was 40 min.

Key words: immune magnetic namoparticles, Salmonella enteritidis, enrichment and separation

CLC Number:

R155.5

HU Yuxin, ZHENG Shu, HE Zao, LUO Fang, LIU Xia. Enrichment and Isolation of Salmonella enteritidis by Immune Magnetic Nanoparticles[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201613029.

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