Rapid and Simultaneous Detection of 4 Milk Oligosaccharides Using LC-MS

doi:10.7506/spkx1002-6630-201614015

Abstract

Abstract:

An ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was used to
rapidly and simultaneously detect 4 oligosaccharides in bovine milk and human milk. Samples were defatted, deproteined,
resolved on an ACQUITY BEH amide column using gradient elution with 0.1% ammonia water/acetonitrile as mobile
phase, detected by mass spectrometric specific ion monitoring and quantified. The standard curves of 2 fucosyllactose
oligosaccharides were linear within the range from 0.78 to 50.00 mg/L with R2 > 0.990. The standard curves of 2
sialyllactose were linear within the range from 1.56 to 100.00 mg/L with R2 > 0.990. The recoveries of spiked samples
ranged from 80.00% to 100.00%. The relative standard deviation (RSD) of repeatability and precision was less than 5.00%.

Key words: ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), oligosaccharides, bovine milk, human milk

CLC Number:

TS207.3

WEI Jinghua, CHEN Lijun, ZHAO Junying, ZHANG Yi, JING Mengna, WANG Pin, JIANG Tiemin, QIAO Weicang. Rapid and Simultaneous Detection of 4 Milk Oligosaccharides Using LC-MS[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201614015.

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