Simultaneous Determination of Five Vitamins by Titania-Based RP-HPLC

doi:10.7506/spkx1002-6630-201614020

Abstract

Abstract:

A new method for simultaneous separation and determination of five vitamins including nicotinic acid (vitamin
PP), biotin (VB7), nicotinamide (vitamin PP), folic acid (VB9), and cyanocobalamin (VB12) using high performance liquid
chromatography (HPLC) on a titania-based column has been developed. The influence of buffer pH, buffer type, buffer
concentration, column temperature and flow rate on separation efficiency was investigated. The optimized chromatographic
conditions were obtained as follows: 5.0 mmol/L phosphate solution at pH 7.0 as buffer solution, column temperature of
50 ℃, and a flow rate of 0.8 mL/min. Biotin and cyanocobalamin were detected at 210 nm and three other vitamins at
270 nm. The thermodynamic parameters enthalpy, entropy and Gibbs free energy were calculated for the retention of the
analytes. The proposed method presented good linearity (R2 > 0.999 0) for the five vitamins. The limits of detection (LODs)
for nicotinic acid, biotin, nicotinamide, folic acid and cyanocobalamin were 6, 10, 20, 22, and 8 ng/mL, respectively. The
precisions (RSDs) for the five vitamins were less than 1.36%. The recoveries of spiked samples were between 92.30% and
107.20%. The precision and accuracy of this method can meet the requirements of HPLC analysis.

Key words: titania-based reverse phase, high performance liquid chromatography (HPLC), vitamins

CLC Number:

TS218

GENG Ying, LI Rong, JIANG Zitao, ZHANG Fabo. Simultaneous Determination of Five Vitamins by Titania-Based RP-HPLC[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201614020.

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