Determination of Aliphatic Aldehydes in Waste Cooking Oil by Pre-Column Derivatization-HPLC Method

doi:10.7506/spkx1002-6630-201614028

Abstract

Abstract:

By taking advantage of lipid peroxidation of unsaturated fatty acids and consequent generation of a variety of
highly reactive small molecular aliphatic aldehydes during the refining of waste cooking oil, a simple and highly sensitive
method was established based on the precolumn derivatization of aliphatic aldehydes utilizing 2,4-dinitrobenzene hydrazine
(DNPH) followed by high performance liquid chromatography (HPLC). Further, high sensitivity was obtained by adding
2-picoline borane (2-PB) as a reducing agent to convert cis- and trans-isomers into the same structure. Compared with edible
oil, the contents of formaldehyde and acetaldehyde in refined waste oil increased significantly, providing an indicator to
discriminate waste cooking oil. This method is specific, highly sensitive and accurate and greatly improves the procedure for
detection and identification of waste cooking oil.

Key words: waste cooking oil, aliphatic aldehyde, 2,4-dinitrobenzene hydrazine, high performance liquid chromatography (HPLC)

CLC Number:

TS229

SHEN Weiyang, WANG Jieqiong, WANG Wan, YU Qingfeng, XIAO Ying. Determination of Aliphatic Aldehydes in Waste Cooking Oil by Pre-Column Derivatization-HPLC Method[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201614028.

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