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Effect of Glycyrrhiza Polysaccharide on the Growth of Listeria monocytogenes in Vitro and in Vivo

LIAO Chengshui1,2, JIA Yanyan1, LI Zhili3, SUN Ningna4, LI Liangwei5, WANG Xiaoli1,5, YU Zuhua1, YU Chuan1, CHENG Xiangchao1,*   

  1. 1. Animal Disease and Public Security Academician Workstation of Henan Province, Henan Key Laboratory of Animal Disease and
    Public Health, Henan University of Science and Technology, Luoyang 471003, China; 2. Key Laboratory of Zoonosis, Ministry of
    Education, Institute of Zoonosis, Jilin University, Changchun 130062, China; 3. College of Food and Bioengineering,
    Henan University of Science and Technology, Luoyang 471023, China; 4. Office of Ruzhou Committee of Communist Party of
    China, Ruzhou 467500, China; 5. Ruzhou Animal Health Inspection Institute, Ruzhou 467500, China
  • Online:2016-09-15 Published:2016-09-22

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Abstract:

Objective: To investigate the effect of Glycyrrhiza polysaccharide on Listeria monocytogenes (Lm) in vitro and
in vivo. Methods: Colony counting method and fluorescence microscope/spectrophotometric method were used to evaluate
the effect of Glycyrrhiza polysaccharide on the growth and biofilm formation of Lm, respectively. The median lethal
dosage (LD50) was calculated by Bliss method. Protective efficacy against 100 × LD50 Lm was tested in mice administered
with Glycyrrhiza polysaccharide at high, middle and low doses. Mice infected with 0.01 × LD50 Lm were killed at 1,
7, 14, 21, 28 and 35 days for the detection of bacterial distribution in spleen, liver, and lung. Results: The promotion
effect of Glycyrrhiza polysaccharide on the growth and biofilm formation of Lm 10403S at low concentrations (10, 20 and
50 μg/mL) was observed in a concentration-dependent manner, whereas it was slightly reduced at high doses (100 and
200 μg/mL). LD50 of Lm was 2.70 × 108 CFU. A total of 50% mice infected with 100 × LD50 Lm could still be alive after
administration of Glycyrrhiza polysaccharide (100 mg/kg). Bacterial number in spleen, liver, and lung of the mice infected
with 0.01 × LD50 Lm was gradually decreased by increasing the dose of Glycyrrhiza polysaccharide. Conclusion: Glycyrrhiza
polysaccharide at low doses can promote the growth of Lm in vitro, but this effect gradually decreases with the increase in
drug concentration. Conclusively, Glycyrrhiza polysaccharide has a protective effect against Lm infection in mice in vivo.

Key words: Glycyrrhiza polysaccharide, Listeria monocytogenes, biofilm formation, inhibition

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