Abstract:

Digital polymerase chain reaction (dPCR), which emerges as a new absolute DNA quantification technique based
on monomolecular target gene amplification, can promote the development and application of modern molecular biology
in accurate quantitative detection. A review of the literature on commercial dPCR applied in food safety detection is given
in this paper, including quantitative detection of genetically modified organisms, animal derived ingredients and foodborne
pathogenic microorganisms. A discussion on the solution of technical problems using dPCR is provided as well. Moreover,
future prospects for the development of dPCR technology in food safety detection are proposed.

Key words: digital polymerase chain reaction (dPCR), food safety, absolute quantification, detection