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Effect of Ultra-High Pressure Treatment on Tropomyosin Conformation in Litopenaeus vannamei

HU Zhihe, ZHANG Qingqing, WU Zijian, XUE Lu, JIA Ying, WANG Xingxuan   

  1. Tianjin Key Laboratory of Food Biotechnology, School of Biotechnology and Food Science,Tianjin University of Commerce, Tianjin 300134, China
  • Online:2016-12-15 Published:2016-12-21

Abstract: The objective of this paper was to explore the effect of ultra-high pressure treatmnet on tropomyosin (TM)
conformation in Litopenaeus vannamei. After being treated under different conditions (pressure: 0.1–800 MPa; holding
time: 10–40 min; temperature: 10–37 ℃), the secondary and tertiary structure of TM was tested by circular dichroism (CD)
spectroscopy and fluorospectrophotometry, and the flexible region was predicted using DNA Star Protean software. Results
showed that the secondary structure of TM did not change significantly, while the tertiary structure significantly changed
after ultra-high pressure treatment. In the pressure range tested, the number of surface-exposed hydrophobic amino acids in
TM was the smallest at 300 MPa, and was the largest at 700 MPa. The flexible region in TM accounted for 72.9% of the total
amino acid sequence. Therefore, the secondary structure of TM was stable and the tertiary structure was unstable after ultrahigh
pressure treatment. The tertiary structure change of TM may be related to its higher proportion of flexibile region.

Key words: tropomyosin, ultra-high prssure treatment, protein conformation, circular dichroism spectroscopy, fluorospectrophotometry

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