Optimization of Purification by Ammonium Sulfate Precipitation of the Major Allergen Tropomyosin from Shrimp (Litopenaeus vannamei)

doi:10.7506/spkx1002-6630-201718030

Abstract

Abstract: Tropomyosin (TM), the major shrimp allergen, is one of the serious food safety problems throughout the world and has drawn much attention. It has been reported that the isolation and purification of TM is of very importance in the identification and control of allergy. In the present study, tropomyosin was purified from Litopenaeus vannamei by an improved method. The results indicated that TM could be purified effectively by adjusting the extraction buffer to pH 6.5 to 7.5 and then adding ammonium sulfate to approximately 30% saturation. The concentration of TM was about 43 μg/μL, as determined by a BCA Protein Assay Kit. The improved method avoided further purification of TM using high performance liquid chromatography (HPLC) or another chromatographic technology. In addition, the process of TM isolation was simplified and TM loss and dilution were avoided during further purification. High concentration of purified TM solution obtained in the present study could be directly used to study its physicochemical properties and allergenicity without being freeze-dried.

Key words: allergen, Litopenaeus vannamei, tropomyosin (TM), isolation, purification

CLC Number:

TS254.1

FU Linglin, FU Shujie, WANG Yanbo, WANG Chong, ZHANG Yan, WU Qi, MA Aijin. Optimization of Purification by Ammonium Sulfate Precipitation of the Major Allergen Tropomyosin from Shrimp (Litopenaeus vannamei)[J]. FOOD SCIENCE, 2017, 38(18): 187-192.

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Optimization of Purification by Ammonium Sulfate Precipitation of the Major Allergen Tropomyosin from Shrimp (Litopenaeus vannamei)

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