Abstract: Illumina HiSeq high throughput sequencing and the traditional plate culture method were comparatively applied to investigate the structure of bacterial community attached to oyster. Results showed that the dominant bacteria attached to oyster belonged to the Gammaproteobacteria (72.3%) class in the Proteobacteria (77.5%) phylum. At the order level, Vibrionales (49.3%) and Alteromonadales (12.2%) were dominant. At the family level, Vibrionaceae (36.8%), Shewanellaceae (10.3%) and Pseudoalteromonadaceae (7.2%) were dominant. At the genus level, the relative proportions of Vibrio (28.3%), Shewanella (10.3%) and Pseudoalteromonas (7.2%) were high. The results of high throughput sequencing for the top three most dominant genera were consistent with those obtained with the traditional culture method although the proportions of these bacteria were somehow different.

Key words: high throughput sequencing, oyster, microbial flora