Abstract: An analytical method based on ultra performance liquid chromatography-tandem mass spectrometry (UPLCMS/ MS) was developed for the determination of eugenol in fish and farming water samples. Fish samples were extracted with n-hexane and the extract was cleaned up by solid phase extraction with a hydrophilic-lipophilic balance (HLB) cartridge. Water samples were directly concentrated and purified with HLB cartridge. Eugenol was quantitated with an internal standard method in the multiple reaction monitoring mode using negative electrospray ionization within 4 min. The calibration curve for the analyte was linear (R2 > 0.996) in the range of 1 to 200 μg/L. The recoveries of the drug from fish and farming water samples were 88.6%–106% and 89.2%–96.5%, respectively with relative standard deviations (RSDs) of 7.82%–11.90% and 5.18%–7.83%. The limits of quantity (LOQs) were 2.5 μg/kg in fish and 0.05 μg/L in water, respectively.

Key words: ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), solid phase extraction, eugenol, fish, farming water