FOOD SCIENCE ›› 2021, Vol. 42 ›› Issue (5): 122-128.doi: 10.7506/spkx1002-6630-20200228-321

• Nutrition & Hygiene • Previous Articles     Next Articles

Protective Effect of Squid Gonadal Phospholipids on MPTP-Induced Zebrafish Model of Parkinson’s Disease

DONG Junfan, SONG Yang, JI Xiuna, LI Xiaobin, LIU Kechun, JIN Meng   

  1. (1. Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250103, China;2. School of Bioengineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, China)
  • Online:2021-03-15 Published:2021-03-29

Abstract: Purpose: To explore whether squid gonadal phospholipids have an inhibitory effect on Parkinson’s disease (PD) induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). Methods: Zebrafish embryos at 1 day post fertilization (dpf) were dechorionated and then divided into different groups including control, 50 μmol/L MPTP, and 50 μmol/L MPTP combined with different concentrations (3, 10 and 20 μg/mL) of squid gonadal phospholipids. Transgenci zebrafish Vmat:GFP and Fli1:GFP at 4 dpf were used to observe and record the development of dopaminergic neurons and brain blood vessels, respectively. At 5 dpf, we utilized the Zebrabox video-tracking system to assay the behavioral changes of wild-type AB zebrafish. Meanwhile, we analyzed the expression of PD-related genes including those encoding alpha-synuclein (α-syn), E3 ubiquitin protein ligase (parkin), PTEN-induced putative kinase 1 (pink1), and tyrosine hydroxylase (th) by real-time fluorescence quantitative polymerase chain reaction. Results: Compared with the control group, zebrafish treated with MPTP exhibited loss of dopaminergic neurons, injured blood vessels in the brain, PD-like behavior, and abnormal expression of PD-related genes. In comparison to the MPTP treatment group, MPTP combined with squid gonadal phospholipids showed a decrease in the percentage dopamine neuron loss, and relieved blood vessel damage in the brain, PD-like behavior, and the abnormal expression of PD-related genes.

Key words: squid gonadal phospholipid; 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine; zebrafish; Parkinson’s disease

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