Optimization of Microwave-Assisted Immobilization of Trypsin Using Response Surface Methodology

doi:10.7506/spkx1002-6630-201706018

Abstract

Abstract: Trypsin was immobilized onto epoxy resin under microwave irradiation. Using one-factor-at-a-time method and response surface methodology (RSM), the optimal conditions of immobilization were obtained as follows: trypsin dosage, 41 mg/g; microwave irradiation time, 10 min; pH, 6.2; and temperature, 30 ℃. Under these conditions, the activity of immobilized trypsin was 322.56 U/g resin, which was higher than that of traditional immobilized trypsin and the immobilization time was shortened from 20 h to a few minutes. Furthermore, immobilized trypsin had a good stability. Thus, microwave-assisted immobilization provides an efficient way of immobilizing trypsin.

Key words: immobilized trypsin, epoxy resin, microwave, response surface methodology, high efficiency

CLC Number:

TS201.2

LIU Weihua, LI Rong, JIANG Zitao. Optimization of Microwave-Assisted Immobilization of Trypsin Using Response Surface Methodology[J]. FOOD SCIENCE, 2017, 38(6): 117-122.

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Optimization of Microwave-Assisted Immobilization of Trypsin Using Response Surface Methodology

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