FOOD SCIENCE ›› 2019, Vol. 40 ›› Issue (2): 310-317.doi: 10.7506/spkx1002-6630-20171020-216

• Safety Detection • Previous Articles     Next Articles

Determination of 4 Pseudo Proteins in Milk and Dairy Products by Magnetic Molecularly Imprinted Polymer Extraction-Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry

SHAN Yi1,2, WANG Xiangxin1,2, CHEN Meijun1, JIANG Yujun1,2,3, MAN Chaoxin1,2,3, MA Wei4,*   

  1. (1. Heilongjiang Green Food Science Research Institute, Northeast Agricultural University, Harbin 150028, China;2. Key Lab of Dairy Science, Ministry of Education, School of Food Science, Northeast Agricultural University, Harbin 150030, China;3. Synergetic Innovation Center of Food Safety and Nutrition, Harbin 150028, China;4. Dongning Entry-Exit Inspection and Quarantine Bureau, Dongning 157200, China)
  • Online:2019-01-25 Published:2019-01-22

RichHTML

11

PDF (PC)

89

Abstract: A simple and rapid method for the determination of 4 pseudo proteins including melamine, cyromazine, dicyandiamide and biuret in milk and dairy products has been developed by using magnetic molecularly imprinted polymers (MMIPs) extraction combined with ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The MMIPs used for the extraction of 4 pseudo proteins were synthesized by polymerization using biuret-13C2 and cyromazine-D4 as templates and Fe3O4 magnetite as magnetic component. Under the optimized conditions, good linearity was observed for all target analytes in the concentration range of 5–200 ng/mL with correlation coefficient above 0.999. The limits of detection (LODs) for melamine, cyromazine, dicyandiamide and biure were 0.10, 0.02, 0.05 and 0.50 μg/kg in milk samples and 0.25, 0.05, 0.13 and 1.25 μg/kg in powered milk samples, respectively. The recoveries were between 80.5% and 96.1% with relative standard deviations (RSDs) between 1.1% and 9.2%. The developed method was applied with good precision and accuracy to determine real dairy samples.

Key words: magnetic molecularly imprinted polymers, pseudo protein, ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS)

CLC Number: