Inactivation and Mechanism of Bacillus subtilis NG-2 and Its Spores by Pulsed Light

doi:10.7506/spkx1002-6630-201723012

Abstract

Abstract: Pulsed light was used for the inactivation of Bacillus subtilis NG-2 and its spores. Morphological characteristics of the irradiated cells and spores were explored by scanning electron microscope and transmission electron microscope, the leakage of intracellular contents was determined by ultraviolet spectrophotometry, and intracellular calcium ion concentration and nonspecific esterase activity were investigated by fluorescence spectroscopy. The results showed that the optimal pulsed light parameters were determined as 5 cm and 5 cycles/s for distance and flash frequency, respectively. Under the optimized conditions, the total number of bacterial colonies was killed by more than 50% and 99.98% at 15 and 40 s of irradiation time, respectively. The structures of the cell and spore wall were damaged, and intercellular nucleic acid, proteins, diethyl 2,6-pyridinedicarboxylate, Ca2+ and non-specific esterase activity were decreased after irradiation. The findings may provide a theoretical basis for the industrial application of pulsed light.

Key words: pulsed light, Bacillus subtilis, spore, inactivation mechanism

CLC Number:

TS201.3

GUAN Feng, XU Wenchai, YUAN Yongjun, ZHANG Ruixue, ZHAO Simin, CHEN Qiuping. Inactivation and Mechanism of Bacillus subtilis NG-2 and Its Spores by Pulsed Light[J]. FOOD SCIENCE, 2017, 38(23): 70-74.

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Inactivation and Mechanism of Bacillus subtilis NG-2 and Its Spores by Pulsed Light

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