Serotyping, Virulence Genes and Pulsed Field Gel Electrophoresis (PFGE) Typing of Staphylococcus aureus Isolated from Fresh Milk

doi:10.7506/spkx1002-6630-20180110-124

Abstract

Abstract: Objective: To investigate the serotyping, virulence genes and pulsed field gel electrophoresis (PFGE) typing of Staphylococcus aureus in fresh milk for a better understanding of the epidemiological characteristics of S. aureus. Methods: S. aureus strains were isolated from fresh milk samples collected from dairy farms in four regions of Henan province and they were identified by PCR amplification of the thermostable nuclease gene (nuc). The capsular polysaccharide (Cap) serotypes and virulence genes were determined by PCR. The correlation and genetic relatedness among these S. aureu isolates were analyzed by PFGE. Results: A total of 80 S. aureus strains were isolated from 350 fresh milk samples, with a separation rate of 22.86%. Cap5 (60%) was found to be a popular serotype. Of the positive isolates, 62 (77.5%) carried virulence genes, and the prevalence of the virulence genes set, hlb, hld, lukED, ebp, clfA and clfB were 40.00%, 51.25%, 57.50% , 60.00%, 58.75%, 57.50% and 58.75%, respectively. In addition, 47 (58.75%) strains carried at least 6 virulence genes, set-hla-hlb-hld-lukED-cna-ebp-clfA-clfB were found to be the prevalent virulence genotypes. PFGE profiles of 72 strains were obtained. Based on a 90% similarity, they were divided into 12 clusters and 46 PFGE types. The strains in clusters D, G and J were detected to carry some virulence genotypes, suggesting that virulence genes widely existed in a variety of PFGE types of S. aureus isolated from fresh milk in Henan. Conclusions: Fresh milk samples were contaminated by S. aureu to a certain degree. Most of the isolates carried virulence genes, and the virulence genotypes were complicated, posing a potential threat to the health of consumers. The PFGE subtypes were mainly transmitted in the form of clones, and the clone types were diversified and differentiated. Therefore, further clinical studies are needed for serotyping, virulence gene detection and molecular typing of S. aureus in raw milk and dairy products.

Key words: milk, Staphylococcus aureus, serotype, virulence genes, PFGE

CLC Number:

TS201.3

LIU Baoguang, CAI Tian, LI Xiaoshen, LIU Yingying, HE Dandan, KUANG Xiuhua, GAO Yanling, HU Gongzheng. Serotyping, Virulence Genes and Pulsed Field Gel Electrophoresis (PFGE) Typing of Staphylococcus aureus Isolated from Fresh Milk[J]. FOOD SCIENCE, 2019, 40(2): 281-286.

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Serotyping, Virulence Genes and Pulsed Field Gel Electrophoresis (PFGE) Typing of Staphylococcus aureus Isolated from Fresh Milk

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