FOOD SCIENCE ›› 2019, Vol. 40 ›› Issue (8): 175-179.doi: 10.7506/spkx1002-6630-20180201-014

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On-line Screening for and Identification of Antioxidant Compounds from Burdock Roots (Arctium lappa L.) by High Performance Liquid Chromatography-Quadrupole Time-of-Flight Mass Spectrometry

ZHENG Zhenjia, ZHANG Ruiling, ZHANG Minmin, QIU Zhichang, ZHANG Bin, QIAO Xuguang   

  1. 1. Key Laboratory of Food Processing Technology and Quality Control in Shandong Province, College of Food Science and Engineering, Shandong Agricultural University, Tai’an 271018, China; 2. Shandong Analysis and Test Center, Key Laboratory of Traditional Chinese Medicine Quality Control Technology, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, China
  • Online:2019-04-25 Published:2019-05-05

Abstract: A new method based on high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-DAD-Q-TOF-MS) was developed for the rapid screening for and identification of antioxidant compounds in burdock root extract. The sample was divided into two parts by the first HPLC; one part flowed through the second HPLC with the 1,1-diphenyl-2-picrylhydrazyl (DPPH) solution for on-line screening for the antioxidants; the other was flowed through DAD-Q-TOF-MS for on-line rapid identification. Caffeic acid and its 18 derivatives were identified by their relative molecular masses as determined by high resolution MS in the negative ion mode and by comparison of their UV absorption characteristics with literature data and reference standards. Overall, this method is a powerful tool for rapid screening for and identification of antioxidant compounds from complicated systems such as foods and Chinese traditional medicinal materials.

Key words: burdock (Arctium lappa L.), antioxidant compounds, on-line screening, identification, HPLC-DAD-Q-TOF-MS

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