Abstract: This study aimed at the extraction, fractionation and functional characterization of proteins from radish seed meal. According to their solubility, albumin, globulin, glutelin and prolamin were separated from radish seed meal. The amino acid composition of the protein isolate extracted from radish seed meal was determined with an automatic amino acid analyzer and its functional properties and 1,1-diphenyl-2-picrylhydrazyl free radicals, ·OH, NO2 - and H2O2 scavenging capacity of in vitro were analyzed by physical and chemical methods. Results showed that globulin, glutelin, albumin and prolamin accounted for 44%, 33%, 21% and 2% of the total proteins in radish seed meal. Eighteen amino acids were detected in the protein isolate, and the first limiting amino acid was methionine with an amino acid score of 57. From this study, we concluded that radish seed meal proteins have good functional properties and antioxidant capacity and thus can be regarded as a potentially valuable resource that can be developed into high-value products.

Key words: radish seed meal, protein, amino acid composition, functional properties, antioxidant capacity