Abstract: This study was done to analyze the chemical constituents of Raphanus sativus L. seeds for exploring the material basis for consumption of raw Raphanus sativus L. seeds. Sixteen compounds were isolated and purified by chromatography on macroporous resin HP-20, high-porosity polystyrene (MCI gel), ODS, silica gel and LiChroprep RP-18 columns. Their structures were identified by spectroscopic methods as cis-13-docosenoic acid (1), stigmasta-4-en-3-one (2), (22E,24R)-ergosta-5,22-dien-3β-ol (3), β-stigmast-5-en-3β-ol (4), trans-methyl ferulate (5), trans-methyl sinapate (6), β-sitosterol-3-O-β-D-glucoside (7), α-D-galactopyranosyl-(1-6)-α-D-glucopyranosyl-(1-2)-β-D-fructofuranoside) (8), β-D-fructofuranosyl-α-D-(6-O-sinapoyl)-glucopyranoside (9), β-D-(3-O-sinapoyl)-fructofuranosyl-α-D-glucopyranoside (10), β-D-(3-O-sinapoyl)-fructofuranosyl-α-D-(6-O-sinapoyl)-glucopyranoside (11), β-D-(3,4-O-disinapoyl)-fructofuranosyl-α-D-(6-O-sinapoyl)-glucopyranoside (12), isorhamnetin, 3-O-β-D-glucoside (13), isorhamnetin 3,4’-di-O-β-D-glucoside (14), isorhamnetin, 3-O-β-D-glucoside-7-O-α-L-rhamnose (15) and 3’-O-methyl-(-)-epicatechin 7-O-β-D-glucoside (16), respectively. Among these, compounds 2, 3, 7–10, and 13–16 were isolated from the plant for the first time.

Key words: Raphanus sativus L., steroids, flavanoids, sinapoylsucrose, separation and purification