Abstract: This work aimed to study the effects of high hydrostatic pressure (HHP) and enzymatic hydrolysis on the antigenicity of type I collagen from the skin of Oncorhynchus mykiss. The results indicated that the antigenicity of HHPtreated type I collagen decreased initially and then increased with the increase of pressure. Moreover, the antigenicity of type I collagen was reduced by up to 42.66% after 10 min HHP treatment at 200 MPa and 25 ℃. On the other hand, by comparing the effect of protease papain, α-chymotrypsin and trypsin on reducing collagen antigenicity, we obtained the optimal enzymatic hydrolysis conditions that achieved maximum antigenicity reduction (97.69%) as follows: 45 ℃, pH 8.0, enzyme-to-substrate ratio 1:10. Our results showed that the antigenicity of type I collagen from Oncorhynchus mykiss skin could be reduced by both high hydrostatic pressure and enzymatic hydrolysis and that enzymatic hydrolysis was more effective than high hydrostatic pressure. Thus, these results can provide useful information for allergen reduction.

Key words: type I collagen, high hydrostatic pressure, enzymatic hydrolysis, antigenicity