FOOD SCIENCE ›› 2018, Vol. 39 ›› Issue (13): 205-210.doi: 10.7506/spkx1002-6630-201813030

• Nutrition & Hygiene • Previous Articles     Next Articles

Effect of Lonicera caerulea Anthocyanins on the Expression of LDLR, ABCG1 and ABCA1 Genes in Livers of Hyperlipidemic Rats

YU Wei1,2, ZHANG Guifang2, SONG Xuejian3, ZHEN Jinglong3, CHI Xiaoxing3, WANG Zhenyu4,*   

  1. 1. School of Forestry, Northeast Forestry University, Harbin 150040, China; 2. National Coarse Cereals Engineering Research Center, Heilongjiang Bayi Agricultural University, Daqing 163319, China; 3. College of Food Science, Heilongjiang Bayi Agricultural University, Daqing 163319, China; 4. Department of Food Science and Engineering, Harbin Institute of Technology, Harbin 150001, China
  • Online:2018-07-15 Published:2018-07-09

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Abstract: Objective: To investigate the effect of anthocyanins from the fruits of Lonicera caerulea (LCA) on the gene expression of low density lipoprotein receptor (LDLR), ATP-binding cassette transporter G1 (ABCG1), and ATP-binding cassette transporter A1 (ABCA1) in hyperlipidemic rat liver. Methods: A total of 60 male Wistar rats aged two months were randomly divided into 6 groups: normal diet control (ND, intragastrically administered with 1.2 g/(kg·d mb) of physiological saline), high-fat diet control (HFD, similarly administered with 1.2 g/(kg·d mb) of physiological saline), positive control (intragastrically administered with 10 mg/(kg·d mb) of simvastatin tablets), and low-, middle- and high-dose LCA (at 4.0, 40.0 and 120.0 mg/(kg·d mb) groups. The administration lasted for 28 days. At the end of this period, the levels of serum total cholesterol (TC) and triglyceride (TG), HDL cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), apolipoprotein A (Apo-A) and apolipoprotein B (Apo-B) were determined. The mRNA expression levels of LDLR, ABCA1 and ABCG1 were detected by real-time polymerase chain reaction (RT-PCR) and the protein level of LDLR was evaluated by Western blot analysis. Results: Compared with the hyperlipemia model group, LCA could reduce the serum levels of TC, TG, LDL-C and Apo-B and increase the levels of HDL-C and Apo-A (P < 0.05 or P < 0.01). The expression of LDLR protein and mRNA in all dose groups increased significantly (P < 0.05), and the expression levels of ABCA1 and ABCG1 mRNA similarly rose; in particular, a significant increase was observed in the middle- and high-dose groups (P < 0.05). Conclusion: 40.0 mg/(kg·d mb) Lonicera caerulea anthocyanins have a significant role in regulating blood lipids by up-regulating the expression of LDLR and ABC family genes in the liver and consequently regulating reverse cholesterol transportation in hyperlipidemic rats.

Key words: Lonicera caerulea anthocyan, hyperlipidemic rats, low density lipoprotein receptor, ATP-binding cassette transponter G1, ATP-binding cassette transporter A1

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