FOOD SCIENCE ›› 2018, Vol. 39 ›› Issue (17): 171-176.doi: 10.7506/spkx1002-6630-201817028

• Nutrition & Hygiene • Previous Articles     Next Articles

Effect of Lonicera caerulea Anthocyan on the Role of Cholesterol Metabolism Related Genes in the Liver of Hyperlipidemic Rats

YU Wei1,2, ZHANG Guifang2, ZHEN Jinglong3, SONG Xuejian3, CHI Xiaoxing3, WANG Zhenyu4,*   

  1. 1. School of Forestry, Northeast Forestry University, Harbin 150040, China; 2. National Coarse Cereals Engineering Research Center, Heilongjiang Bayi Agricultural University, Daqing 163319, China; 3. College of Food Science, Heilongjiang Bayi Agricultural University, Daqing 163319, China; 4. College of Food Science and Engineering, Harbin Institute of Technology, Harbin 150001, China
  • Online:2018-09-15 Published:2018-09-18

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Abstract: Objective: The effect of Lonicera caerulea anthocyanin on the expression of liver X receptor α (LXRα), scavenger receptor class B, type I (SR-BI), ATP-binding cassette transporter G5 (ABCG5), peroxisome proliferator-activated receptor γ (PPARγ) and cholesterol 7α-hydroxylase (CYP7a1) in hyperlipidemic rat liver were investigated in this study. Methods: Totally 60 male Wistar rats at the age of two months were randomly divided into 6 groups including 10 rats with normal feed and 50 rats with high-fat diet for the diabetic model establishment. After model establishment in 30 days, the diabetic rats were divided 5 groups with basic diet group (ND, 1.2 g/(kg·d) physiological saline intragastric administration), high-fat diet group (HFD, 1.2 g/(kg·d) physiological saline intragastric administration), 10 mg/(kg·d) simvastatin tablet intragastric administration group as the positive control, and low dose, middle dose and high dose of Lonicera caerulea anthocyanin groups (HFD + L, HFD + M, HFD + H, Lonicera caerulea anthocyanin at 4.0, 40.0 and 120.0 mg/(kg·d). The intragastric administration was last for 28 days. The expression levels of LXRα, SB-BI, ABCG5, PPARγ and CYP7a1 were detected by quantitative real-time polymerase chain reaction (PCR). Results: Compared with HFD group, the mRNA expression levels of SR-BI and ABCG5 mRNA were not significant after the intervention of L. caerulea anthocyanins (P > 0.05); the mRNA expression levels of LXRα and CYP7a1 of HFD + M and HFD + H group in liver tissue were increased (P < 0.05 or P < 0.01); the mRNA expression levels of PPARγ of HFD + L, HFD + M and HFD + H group in liver tissue were decreased. Conclusion: The L. caerulea anthocyan can improve LXRα and CYP7a1 and can decrease PPARγ in liver tissue of hyperlipidemic rats; and regulate blood lipid level of hyperlipidemic rats, thereby preventing arteriosclerosis.

Key words: Lonicera caerulea, Lonicera caerulea anthocyan, hyperlipidemic rats, liver X receptor α, scavenger receptor class B, type I, ATP-binding cassette transporter G5, peroxisome proliferator-activated receptor γ, cholesterol 7α-hydroxylase

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