Mapping of IgG-Binding Epitopes on the A2 Chain of the Major Soybean Allergen 11S Globulin G2

doi:10.7506/spkx1002-6630-201818024

FOOD SCIENCE ›› 2018, Vol. 39 ›› Issue (18): 152-158.doi: 10.7506/spkx1002-6630-201818024

• Bioengineering • Previous Articles Next Articles

Mapping of IgG-Binding Epitopes on the A2 Chain of the Major Soybean Allergen 11S Globulin G2

LIU Yangxingyue, ZHANG Di, YAO Yaya, MIAO Ziye, LIU Zhuang, LI Huijing*

(College of Food Science and Technology, Hebei Agricultural University, Baoding 071000, China)

Online:2018-09-25 Published:2018-09-18

Abstract

Abstract: Adopting bioinformatics software, this study aimed to predict the secondary and tertiary structure model of the A2 chain of 11S globulin G2 in order to locate the B cell epitopes. The results indicated that the conformational epitopes were mainly at the random coil areas and the probable linear epitopes may contain 37KPDNRIE43, 79PSYTNGP85, 114SQQRGRSQRP123, 52WNPNNK57, 196QQGGSQSQKGKQQE209, 241QGENEEED248, 267RKPQQEEDDDDEEEQ281, and 287TDKGC291. This prediction can be utilized to find out accurate peptides sequences for subsequent peptide preparation and immunization so as to develop a technique to detect soybean allergens for people allergic to soybean foods in China.

Key words: soybean allergen, A2 chain of 11S globulin G2, bioinformatics software, protein secondary structure, tertiary structure model, B-cell epitope

CLC Number:

TS214.2

LIU Yangxingyue, ZHANG Di, YAO Yaya, MIAO Ziye, LIU Zhuang, LI Huijing. Mapping of IgG-Binding Epitopes on the A2 Chain of the Major Soybean Allergen 11S Globulin G2[J]. FOOD SCIENCE, 2018, 39(18): 152-158.

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Mapping of IgG-Binding Epitopes on the A2 Chain of the Major Soybean Allergen 11S Globulin G2

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