FOOD SCIENCE ›› 2018, Vol. 39 ›› Issue (23): 16-21.doi: 10.7506/spkx1002-6630-201823003

• Basic Research • Previous Articles     Next Articles

Accumulation and Distribution of GII.4 Norovirus in Artificially Contaminated Pacific Oyster

SU Laijin1,2,3, MA Liping1, LIU Hui1, ZHOU Deqing1,*   

  1. 1. Pilot National Laboratory for Marine Science and Technology (Qingdao), Laboratory for Marine Drugs and Biological Products, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China; 2. College of Food Sciences and Technology, Shanghai Ocean University, Shanghai 201306, China; 3. Wenzhou Academy of Agricultural Science, Wenzhou 325006, China
  • Online:2018-12-15 Published:2018-12-17

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Abstract: Oysters are one of the most common vectors for the transmission of noroviruses (NoVs), which are one of the most important food-borne viral pathogens worldwide. The distribution of NoVs in oysters varies significantly with seasons and tissues. In order to understand the accumulation and distribution of norovirus in oysters, oysters were farmed under controlled conditions after being artificially contaminated with GII.4 NoV in the laboratory. The contents and distribution of GII.4 NoV in various oyster tissues were detected by real-time quantitative reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemistry. The real-time quantitative RT-PCR results showed that the content of GII.4 NoV remained stable in the range of (8.75 ± 0.36) × 104 to (9.12 ± 0.49) × 104 copies for oyster mantle, but initially increased to a maximum of (1.15 ± 0.15) × 106 copies at 6 h and then decreased for oyster gills. The content of GII.4 NoV in oyster digestive tissues was continuously increased to reach a plateau ((1.06 ± 0.14)×106 copies) at 12 h. GII.4 NoV was mainly accumulated in oyster digestive tissues at 24 h. The content of GII.4 NoV was not significantly changed after purification (P > 0.05). The immunohistochemistry results showed that GII.4 NoV was widely distributed in the mantle edge, the gill wall and the inner wall of digestive tissues. The seasonal distribution of GII.4 NoV in various oyster tissues was in accordance with the real-time quantitative RT-PCR results.

Key words: norovirus, artificial contamination, oyster, accumulation, distribution

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