FOOD SCIENCE ›› 2018, Vol. 39 ›› Issue (24): 183-188.doi: 10.7506/spkx1002-6630-201824028

• Bioengineering • Previous Articles     Next Articles

DNA Fingerprinting of Malt Varieties Using Tailed Primer M13 Microsatellite (TP-M13-SSR) Markers

ZHANG Zhijun, HUANG Kexing, YUE Jie, LIU Mingli, QI Mingxia, DONG Jianjun*   

  1. (State Key Lab of Biological Fermentation Engineering of Beer, Tsingtao Brewery Co. Ltd., Qingdao 266100, China)
  • Received:2018-12-25 Revised:2018-12-25 Online:2018-12-25 Published:2018-12-17

Abstract: The aim of this study was to construct a DNA fingerprint for identifying different malt varieties based on tailed primer M13 microsatellite (TP-M13-SSR) markers. Four simple sequence repeat (SSR) primer pairs with high polymorphisms were successfully screened out from 163 candidates, and an average of 6.75 genotypes were detected using each SSR primer pair, on average with 3-10 alleles. In the case of massive primer screening, the use of M13 universal primers could greatly reduce the cost of fluorescent tags of SSR primers. After analysis of alleles, four selected primer pairs could distinguish 23 malt varieties using specific alleles, and a DNA fingerprint database was established for the different malt varieties investigated in this study. In conclusion, this method was cheap, accurate and reliable, and it could provide technical support for breweries to purchase malt.

Key words: malt, DNA fingerprinting, TP-M13-SSR, varietal identification

CLC Number: