Extraction of Superoxide Dismutase from Tomato Using Amino Functionalized Ionic Liquid

doi:10.7506/spkx1002-6630-20200905-062

FOOD SCIENCE ›› 2021, Vol. 42 ›› Issue (21): 56-62.doi: 10.7506/spkx1002-6630-20200905-062

• Basic Research • Previous Articles Next Articles

Extraction of Superoxide Dismutase from Tomato Using Amino Functionalized Ionic Liquid

WANG Tao, ZHAI Chen, WANG Liang, LI Mengyao, WANG Shuya, XIE Yunfeng

(1. College of Life Science and Technology, Xinjiang University, ürümqi 830017, China; 2. Beijing Key Laboratory of Nutrition & Health and Food Safety, Nutrition & Health Research Institute, COFCO Corporation, Beijing 102209, China)

Online:2021-11-15 Published:2021-11-23

Abstract

Abstract: In this work, an aqueous two-phase system formed by an amino functionalized ionic liquid as a novel green solvent and inorganic salts was employed for extracting superoxide dismutase (SOD) from tomatoes. We investigated the effects of the type and concentration of ionic liquid, K2HPO4 concentration, extraction temperature and extraction time on the activity of SOD. Through the combined use of one-factor-at-a-time method and response surface methodology, the optimal extraction conditions that provided maximum SOD activity (345.68 U/g) were established as follows: concentration [(H2NC2)Mim]Br ionic liquid, 0.40 g/mL; K2HPO4 concentration, 0.60 g/mL; extraction temperature, 35 ℃; and extraction time, 25 min. Compared with the traditional buffer solution method and the [C4Mim]Br/K2HPO4-based aqueous two-phase extraction method, this method yielded higher SOD activity with smaller standard deviations. This research provides a new experimental idea for the extraction of protease substances in fruits and vegetables.

Key words: functionalized ionic liquid; aqueous two-phase extraction; superoxide dismutase activity

CLC Number:

TS255.1

WANG Tao, ZHAI Chen, WANG Liang, LI Mengyao, WANG Shuya, XIE Yunfeng. Extraction of Superoxide Dismutase from Tomato Using Amino Functionalized Ionic Liquid[J]. FOOD SCIENCE, 2021, 42(21): 56-62.

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Extraction of Superoxide Dismutase from Tomato Using Amino Functionalized Ionic Liquid

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