FOOD SCIENCE ›› 2021, Vol. 42 ›› Issue (23): 182-195.doi: 10.7506/spkx1002-6630-20201022-226

• Nutrition & Hygiene • Previous Articles     Next Articles

Mechanism by which 10-Hydroxy-2-decenoic Acid Induces Cell Apoptosis and Cycle Arrest and Inhibits Migration in Hepatocellular Carcinoma HepG2 Cells

ZANG Yanqing, JU Xueying, ZHAI Yuqing, YAO Di, ZHU Lei, WANG Changyuan   

  1. (1. College of Food Science, Heilongjiang Bayi Agricultural University, Daqing 163319, China; 2. National Coarse Cereals Engineering Research Center, Daqing 163319, China)
  • Online:2021-12-15 Published:2021-12-29

Abstract: 10-Hydroxy-2-decenoic acid (10-HDA) is a unique bioactive component of royal jelly. In this study, the molecular mechanism of apoptosis in HepG2 cells induced by 10-HDA was analyzed by cell counting kit-8 (CCK-8) assay, Hoechst 33342/propidium iodide (PI) staining, flow cytometry and Western blot. The results showed that 10-HDA had significant cytotoxicity on liver cancer cell lines but not normal cells (L-02, IMR-90 or GES-1). 10-HDA down-regulated the expression of Bcl-2, up-regulated the expression of Bax , triggered the mitochondrial apoptosis pathway, and decreased mitochondrial membrane potential, thus leading to the release of cytochrom c (Cyt c), the activation of caspase-3, and eventually mitochondria-dependent apoptosis. In addition, 10-HDA induced reactive oxygen species (ROS) accumulation, activated the mitogen-activated protein kinase (MAPK) signaling pathway and the signal transducer and activator of transcription 3 (STAT3) signaling pathway, promoted the expression of jun N-terminal kinase (JNK) and P-P38, and inhibited the expression of extracellular-signal-regulated kinase (ERK) and P-STAT3, consequently regulating the expression of cell cycle related-proteins to arrest the cell cycle at the G2/M phase. Finally, 10-HDA was demonstrated to inhibit cancer cell migration by activating the transforming growth factor-β1 (TGF-β1) signaling pathway. In conclusion, 10-HDA can induce cell apoptosis by causing ROS accumulation and activating the MAPK and STAT3 signaling pathways.

Key words: 10-hydroxy-2-decenoic acid; hepatocellular carcinoma cells; cell apoptosis; cycle arrest; cell migration; signaling pathway

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