FOOD SCIENCE ›› 2021, Vol. 42 ›› Issue (23): 237-244.doi: 10.7506/spkx1002-6630-20201217-207

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Effect of 3-Aminobenzoic Acid Ethyl Ester Methanesulfonate (MS-222) on Improving the Survival of Turbot (Scophthalmus maximus) during Simulated Live Transportation

CAO Jie，XIE Jing   

  1. (1. College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China; 2. National Experimental Teaching Demonstration Center for Food Science and Engineering (Shanghai Ocean University), Shanghai 201306, China; 3. Shanghai Aquatic Products Processing and Storage Engineering Technology Research Center (Shanghai Ocean University), Shanghai 201306, China)
  • Online:2021-12-15 Published:2021-12-29

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Abstract: In order to study the anesthetic effect of 3-aminobenzoic acid ethyl ester methanesulfonate (MS-222) in turbot, simulated transportation experiments were conducted to evaluate the survival rate of turbot under varying conditions of MS-222 concentration (20, 40, 60 and 80 mg/L), water temperature (2, 8, 13 and 20 ℃) and fish to water ratio (2:1, 1:1, 1:3 and 1:5; m/m), and the optimal live transportation conditions were determined. Before this, under still water conditions, the influence of different MS-222 concentrations (20, 40, 60, 80, 100 and 120 mg/L) on the behavior of turbot was explored by determining the anesthesia time, recovery time and survival rate after recovery to determine the effective anesthetic concentration of MS-222 for turbot. Meanwhile, the changes in the ammonia nitrogen concentration in the water and the changes in physiological and biochemical indicators in the serum and muscle of turbot were measured during simulated live transportation. Results showed that a water temperature of 8 ℃, an MS-222 concentration of 40 mg/L and a fish to water ratio of 1:3 were suitable for long-time live transportation (24 h) of turbot. With increasing transportation time, the ammonia nitrogen concentration in the water increased and the dissolved oxygen level decreased. The lactic acid content in the muscle increased, and the glycogen content and pH decreased. The activities of lactate dehydrogenase and aspartate aminotransferase and the levels of glucose, urea and creatinine in the serum changed significantly with transportation time up to 24 hours (P < 0.05), indicating that the liver and kidney functions of turbot were damaged during transportation, and the changes in all biochemical indexes were smaller in the anesthesia group than in the control group. Taken together, the proper application of MS-222 can increase the survival rate of turbot and prolong the live transportation time.

Key words: 3-aminobenzoic acid ethyl ester methanesulfonate; turbot; live transportation; anesthetic effect; physiological and biochemical indexes

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