Abstract: A method for the quantitative determination of ergosterol in edible fungi was established by using deuterated chloroform as the solvent and pyrazine as the internal standard. The C-18H proton single peak at δ 0.63 was selected as the target characteristic peak after optimizing the sampling parameters, acquisition time (AT), the number of scans (NS) and relaxation delay time (D1). The methodological evaluation results showed that the calibration curve was linear in the concentration range of 0.55–5.30 mg/mg with correlation coefficient (R2) of 0.999 7. The limit of detection and the limit of quantitation were 0.001 8 and 0.045 0 mg/mL, respectively. The relative standard deviation (RSD) values for precision and stability were both less than 2% and the average recovery of the added ergosterol was 103.491 9%. The specificity of the developed method was verified by applying it to detect three varieties of edible fungi samples, indicating that this method has the advantage of rapidity and accuracy.

Key words: nuclear magnetic resonance spectroscopy; quantitative detection; edible fungi; ergosterol