Structure Identification and Activity Screening of Active Peptides in Colla Corii Asini (Ejiao)

doi:10.7506/spkx1002-6630-20210531-360

FOOD SCIENCE ›› 2022, Vol. 43 ›› Issue (10): 207-213.doi: 10.7506/spkx1002-6630-20210531-360

• Bioengineering • Previous Articles Next Articles

Structure Identification and Activity Screening of Active Peptides in Colla Corii Asini (Ejiao)

WANG Yingxue, FAN Yumei, LIAO Feng, YU Zhipeng

(1. College of Food Science and Technology, Bohai Univercity, Jinzhou 121013, China;2. National Engineering Research Center for Gelatin-based Traditional Chinese Medicine, Dong’e Ejiao Co. Ltd., Liaocheng 252201, China; 3. School of Food Science and Engineering, Hainan University, Haikou 570228, China)

Online:2022-05-25 Published:2022-05-27

Abstract

Abstract: In this study, liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to identify the structures of peptides in Colla Corii Asini (Ejiao) subjected to simulated gastrointestinal digestion. The peptides were screened for their anti-hypertensive, anti-Alzheimer, and anti-tumor potential by molecular docking. The results showed that 519 active peptides with molecular mass ranging from 661.4 to 2 851.4 Da were obtained from the digested products of Ejiao. These peptides were mainly derived from the enzymatic hydrolysis of collagen and hemoglobin. Peptides KGETGLR, SGLDGAKG, and ADGVAGPK were found to have the highest anti-hypertensive, anti-Alzheimer and anti-tumor potential. Hydrogen bond and electrostatic interactions played a vital role in the anti-hypertensive, anti-Alzheimer, and anti-tumor activities of the Ejiao peptides.

Key words: gastrointestinal digestion; Ejiao active peptides; molecular docking; anti-hypertensive; anti-Alzheimer’s; anti-tumor

CLC Number:

TS201.4

WANG Yingxue, FAN Yumei, LIAO Feng, YU Zhipeng. Structure Identification and Activity Screening of Active Peptides in Colla Corii Asini (Ejiao)[J]. FOOD SCIENCE, 2022, 43(10): 207-213.

[1]	Yu-Ting WANG Fuying Chu Hong Lei. Metabonomics Study of Intestinal Microflora in the Improvement of IDA by Auricularia Auricula Melanin in Mice [J]. FOOD SCIENCE, 0, (): 0-0.
[2]	Zi-Ang TIAN Xiao-Yan ZHENG SHENG Zhan-Wu. Research progress on the Interaction and functional effect of protein - polyphenol - starch [J]. FOOD SCIENCE, 0, (): 0-0.
[3]	Zhengwei Tian Ji-Chun ZHAO JIan Ming. Effect of LAB fermented on broccoli glucosinolates alleviating oxidative stress in Caenorhabditis elegans [J]. FOOD SCIENCE, 0, (): 0-0.
[4]	Xue Li Ji-Ke LU. Comparison of Bioactivities of water extracts from five kinds of edible Mushrooms [J]. FOOD SCIENCE, 0, (): 0-0.
[5]	Qing WANG. The effect of Angelica dahurica polysaccharide on hypoglycemic and hepatoprotective in type 2 diabetic rats [J]. FOOD SCIENCE, 0, (): 0-0.
[6]	xiang li. Fermentation of yeast mannoprotein in vitro and anti-inflammatory activity of its metabolites [J]. FOOD SCIENCE, 0, (): 0-0.
[7]	Hua Fan Fuguo Liu Yinxia Li Liu Xue-Bo. Micronization with alkaline-assisted enzymatic extraction improves the physicochemical and functional properties of soluble dietary fiber in Herba lophatheri [J]. FOOD SCIENCE, 0, (): 0-0.
[8]	SHI Shen, LI Haoqiu, WANG Long, LI Nan, TAN Shengjie, LIU Hua, PENG Guiying, LI Yalan, ZHANG Chong, FENG HAOTIAN. Protective Effects of Three Types of Dairy Products on Immune Vulnerability in Vinorelbine-Treated Zebrafish [J]. FOOD SCIENCE, 2022, 43(11): 113-120.
[9]	FAN Yingrun, ZHENG Tingting, LI Zelin, SHEN Xiaojing, WANG Xuefeng, GU Dahai, XIAO Zhichao, FAN Jiangping. Structural Identification of Leccinum crocipodium (Letellier.) Watliag Polysaccharide and Its Effect on Short Chain Fatty Acid Contents in Cecum Contents and Feces of Mice [J]. FOOD SCIENCE, 2022, 43(11): 130-136.
[10]	LUO Qing, LU Lu, YAN Yamei, MI Jia, LI Xiaoying, CAO Youlong, ZENG Xiaoxiong. Immunomodulatory Effects of Spray Dried Powder of Goji (Lycium barbarum L.) and Goji Polysaccharides on Immunosuppressive Mice Induced by Cyclophosphamide and Their Regulation on Gut Microbiota [J]. FOOD SCIENCE, 2022, 43(11): 137-148.
[11]	WEI Zheng, HE Yan, HUANG Xianzhi, SHEN Yihong, DING Xiaowen. Improving Effect of Tea Extract Rich in Polysaccharides and Polyphenols on Intestinal Oxidative Stress in Mice and Underlying Mechanism [J]. FOOD SCIENCE, 2022, 43(11): 149-155.
[12]	ZHANG Tao, CHEN Yanhong, CHANG Gaoping, YANG Yuanfan, DU Xiping, JIANG Zedong, NI Hui, LI Qingbiao. Understanding the Mechanism of Action of Echinenone on Alzheimer’s Disease from the Perspective of Acetylcholinesterase and Oxidative Stress [J]. FOOD SCIENCE, 2022, 43(11): 105-112.
[13]	Ying-Run FAN Xiao-Jing SHEN. Idetification of Leccinellum crocipodium( Letellier.) Watliag Polysaccharides Structure and its effect on Short Chain Fatty Acid Contents in Cecum Content and Feces of mice [J]. FOOD SCIENCE, 0, (): 0-0.
[14]	Yanan CAO lianxin peng. Research progress on the probiotic effects of plant polysaccharides and its influencing factor [J]. FOOD SCIENCE, 0, (): 0-0.
[15]	. Research progress in the application of high-voltage electric field technology in food sterilization [J]. FOOD SCIENCE, 0, (): 0-0.

Structure Identification and Activity Screening of Active Peptides in Colla Corii Asini (Ejiao)

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments