Isolation, Identification and Biological Characteristics of Bioprotective Bacteria from Vacuum Packaged Chilled Beef

doi:10.7506/spkx1002-6630-20211208-094

FOOD SCIENCE ›› 2022, Vol. 43 ›› Issue (22): 151-158.doi: 10.7506/spkx1002-6630-20211208-094

• Bioengineering • Previous Articles

Isolation, Identification and Biological Characteristics of Bioprotective Bacteria from Vacuum Packaged Chilled Beef

YANG Huixuan, LUO Xin, ZHU Lixian, YANG Xiaoyin, HAN Guangxing, LI He, DONG Pengcheng, ZHANG Yimin

(1. College of Food Science and Engineering, Shandong Agricultural University, Tai’an 271018, China;2. Linyi Station, National Beef Cattle Industrial Technology System, Linyi 276000, China; 3. Tongliao Station, National Beef Cattle Industrial Technology System, Tongliao 028000, China)

Published:2022-12-12

Abstract

Abstract: A total of seven lactic acid bacteria strains with bioprotective potential were isolated and characterized from vacuum-packaged chilled beef. By morphological observation, physiological and biochemical identification and 16S rDNA sequencing, they were determined as Lactobacillus sakei. All isolates grew well at 25?35 ℃, and RS-16 and RS-25 grew better and had a stronger acid-producing ability. Antibiotic resistance and virulence genes were detected in RS-33 and RS-41 but not any of the other strains. The results of agar diffusion test showed that the seven isolates had antagonistic effect on all indicator strains, and the antagonistic effect of strains RS-16 and RS-25 on Salmonella typhimurium was better than that of the other strains (P < 0.05). Organic acids secreted by the isolated strains were found to have antibacterial activity.

Key words: vacuum packaging; chilled beef; isolation and identification; biological protection; Lactobacillus sakei

CLC Number:

TS251.1

YANG Huixuan, LUO Xin, ZHU Lixian, YANG Xiaoyin, HAN Guangxing, LI He, DONG Pengcheng, ZHANG Yimin. Isolation, Identification and Biological Characteristics of Bioprotective Bacteria from Vacuum Packaged Chilled Beef[J]. FOOD SCIENCE, 2022, 43(22): 151-158.

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Isolation, Identification and Biological Characteristics of Bioprotective Bacteria from Vacuum Packaged Chilled Beef

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