A Fluorescent Probe Based on Cu,Fe-N-C Nanozyme with Oxidase-like Activity for Detection of Phthalate Esters

doi:10.7506/spkx1002-6630-20211213-143

Abstract

Abstract: A simple and sensitive fluorescent probe for the determination of phalic acid ester (PAEs) in fermented foods was developed based on the fact that the oxidase-like activity of Cu,Fe-N-C nanozyme can be regulated by PAEs. After PAEs were adsorbed on the surface of Cu,Fe-N-C, the catalytic oxidation of the substrate o-phenylenediamine (OPD) was accelerated, thus increasing the fluorescence intensity of the generated 2,3-diaminophenazine (DAP). The calibration curve for PAEs showed a good linear relationship in the concentration range of 1.25–50 μg/L. The detection limit of the proposed method was 0.76 μg/L. The recoveries of spiked samples ranged from 88.0% to 104.6%, which meet the detection requirements of the national standard GB 5009.271-2016. This method is simple, stable and rapid, and its detection limit is lower than the national limit for PAEs content in foods.

Key words: phthalic acid esters; nanozyme; oxidase-like activity; fluorescent probe

CLC Number:

TS255.7

LI Hong, SHI Qiao, CHEN Junfei, WANG Xinrui, TANG Huihua, LI Qiulan, YANG Dezhi, YANG Yaling. A Fluorescent Probe Based on Cu,Fe-N-C Nanozyme with Oxidase-like Activity for Detection of Phthalate Esters[J]. FOOD SCIENCE, 2023, 44(4): 306-312.

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A Fluorescent Probe Based on Cu,Fe-N-C Nanozyme with Oxidase-like Activity for Detection of Phthalate Esters

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