Progress in the Rapid Detection of Foodborne Pathogens Based on Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-Associated Protein (Cas)

doi:10.7506/spkx1002-6630-20220316-195

FOOD SCIENCE ›› 2023, Vol. 44 ›› Issue (7): 313-323.doi: 10.7506/spkx1002-6630-20220316-195

• Reviews • Previous Articles

Progress in the Rapid Detection of Foodborne Pathogens Based on Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-Associated Protein (Cas)

KOU Xiuying, ZHOU Baoqing, CHEN Ling, DAI Jingsha, ZHANG Jumei, WU Qingping

(1. Infinitus (China) Co., Ltd., Guangzhou 510405, China; 2. State Key Laboratory of Applied Microbiology in Southern China, Guangdong Provincial Key Laboratory of Microbial Safety and Health, Key Laboratory of Agricultural Microbiomics and Precision Application, Ministry of Agriculture and Rural Affairs, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, China)

Published:2023-04-25

Abstract

Abstract: The early screening and rapid detection of foodborne pathogens is critical to food safety and public health. However, the traditional detection method is cumbersome, time-consuming and laborious, and cannot meet the requirement of rapid detection. CRISPR/Cas, composed of clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein (Cas), is an immune system widely present in bacteria and archaea, which provides a novel pathway for low-cost, rapid, specific and sensitive detection of foodborne pathogens due to its efficient and specific sequence recognition and cleavage activity. This review article introduces the principle, mechanism and development of the CRISPR/Cas system, and summarizes the latest progresses in the rapid detection of foodborne pathogens based on the CRISPR/Cas system combined with different result reporting methods in recent years. Its advantages, limitations and future development prospects are also discussed.

Key words: clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein; foodborne pathogens; trans-cleavage; rapid detection technology

CLC Number:

Q93-331

KOU Xiuying, ZHOU Baoqing, CHEN Ling, DAI Jingsha, ZHANG Jumei, WU Qingping. Progress in the Rapid Detection of Foodborne Pathogens Based on Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-Associated Protein (Cas)[J]. FOOD SCIENCE, 2023, 44(7): 313-323.

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Progress in the Rapid Detection of Foodborne Pathogens Based on Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-Associated Protein (Cas)

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