Abstract: According to the hierarchical structure of the human skin, bionic skin microtissue with epidermal layer and subcutaneous tissue layer, which can simulate the reaction of the skin to food allergens, was bioprinted with human skin fibroblasts and RBL-2H3 cells and was used to construct a bionic skin electrochemical sensor by combined use of polypyrrole modification and rapid electrochemical detection technology. The results showed that the sensor could identify egg albumin well, and the linear range was 0.5–2.5?μg/mL. A linear regression equation was fitted as follows: y = 4.45C + 14.24, with a correlation coefficient (R2) of 0.998, and the detection limit of this method was 0.19?μg/mL. The successful construction of the electrochemical sensor shows the possibility of applying biological 3D printing technology to prepare multi-cell composite bionic structures.

Key words: bionic skin; electrochemical sensor; allergen detection