FOOD SCIENCE ›› 2023, Vol. 44 ›› Issue (12): 217-224.doi: 10.7506/spkx1002-6630-20220719-216
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LIU Jianlei, SHANG Bo, XING Xiaoting, ZHANG Dong, CHANG Liu, SUN Hui, DUAN Xiaoliang
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Abstract: In this study, the amylose contents in 24 commercial foxtail millet samples from major production provinces in China were determined by four different methods, namely, the improved national standard method (GB/T 15683-2008), the spectrophotometric method (NY/T 2639-2014) recommended by China’s agricultural authority, the dual-wavelength method, and the concanavalin A (Con A) method. The results of these methods were in the following decreasing order: improved GB/T 15683-2008 ≈ dual-wavelength > NY/T 2639-2014 > Con A. The correlation coefficient between the results of improved GB/T 15683-2008 and those of NY/T 2639-2014 was highest, 0.752 (P = 0), the former being about 1.41 times higher than the latter, which could allow simple prediction of the amylose content of foxtail millet. In conclusion, the improved GB/T 15683-2008 method, the dual-wavelength method and the NY/T 2639-2014 (× 1.41) method can be used to accurately determine the content of amylose in foxtail millet while greatly reducing sample testing time.
Key words: foxtail millet; amylose content; colorimetry; dual-wavelength method; concanavalin A
CLC Number:
TS237
LIU Jianlei, SHANG Bo, XING Xiaoting, ZHANG Dong, CHANG Liu, SUN Hui, DUAN Xiaoliang. Comparison of Four Methods for the Determination of the Amylose Content in Foxtail Millet[J]. FOOD SCIENCE, 2023, 44(12): 217-224.
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URL: https://www.spkx.net.cn/EN/10.7506/spkx1002-6630-20220719-216
https://www.spkx.net.cn/EN/Y2023/V44/I12/217