Heterologous Expression and Enzymatic Properties of Nicotinamide Nucleoside Kinase from Saccharomyces cerevisiae

doi:10.7506/spkx1002-6630-20220912-095

FOOD SCIENCE ›› 2023, Vol. 44 ›› Issue (16): 192-197.doi: 10.7506/spkx1002-6630-20220912-095

• Bioengineering • Previous Articles Next Articles

Heterologous Expression and Enzymatic Properties of Nicotinamide Nucleoside Kinase from Saccharomyces cerevisiae

HE Jianju, LIU Xinxin, SHI Hongling, RAN Luni, WANG Xian, ZHANG Yingjun, TANG Cunduo

(1. College of Life Science and Agricultural Engineering, Nanyang Normal University, Nanyang 473061, China; 2. Postdoctoral Innovation Practice Base, She Dian Lao Jiu Co. Ltd., Nanyang 473300, China; 3. College of Food Science and Technology, Henan Agricultural University, Zhengzhou 450002, China)

Online:2023-08-25 Published:2023-09-01

Abstract

Abstract: In this study, the nicotinamide riboside kinase 1 gene (ScNRK1) was cloned from Saccharomyces cerevisiae, and its soluble expression in Escherichia coli was achieved using pET28a plasmid. The enzyme activity in the fermentation broth was 14.75 IU/mL, and the specific activity the purified enzyme was 2 252.59 IU/mg. In addition, the kinetic parameters of ScNRK1 were significantly higher than those of other reported nicotinamide nucleoside kinases, so ScNRK1 could be more advantageous in the enzymatic synthesis of nicotinamide mononucleotide (NMN).

Key words: nicotinamide nucleoside kinase; β-nicotinamide mononucleotide; expression; identification; biocatalysis

CLC Number:

Q814.9

HE Jianju, LIU Xinxin, SHI Hongling, RAN Luni, WANG Xian, ZHANG Yingjun, TANG Cunduo. Heterologous Expression and Enzymatic Properties of Nicotinamide Nucleoside Kinase from Saccharomyces cerevisiae[J]. FOOD SCIENCE, 2023, 44(16): 192-197.

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Heterologous Expression and Enzymatic Properties of Nicotinamide Nucleoside Kinase from Saccharomyces cerevisiae

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