Screening and Identification of Probiotics with in Vitro Anti-Allergic Activity Based on the Degranulation Inhibition of RBL-2H3 Cells

doi:10.7506/spkx1002-6630-20230613-098

FOOD SCIENCE ›› 2024, Vol. 45 ›› Issue (6): 55-63.doi: 10.7506/spkx1002-6630-20230613-098

• Bioengineering • Previous Articles Next Articles

Screening and Identification of Probiotics with in Vitro Anti-Allergic Activity Based on the Degranulation Inhibition of RBL-2H3 Cells

Ma Ding, Qin Shuangxia, Hao Huichao, Deng Fangming, Zhao Lingyan

(1. College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China;2. Changsha Innovation Institute for Food, Hunan Agricultural University, Changsha 410128, China)

Online:2024-03-25 Published:2024-04-03

Abstract

Abstract: To investigate the anti-allergic activity of lactic acid bacteria, lactic acid bacterial strains were obtained from fermented vegetables by the traditional isolation technique and screened for in vitro allergic activity by the degranulation inhibition assay against RBL-2H3 cells. The results showed that all four isolates exhibited good probiotic properties and safety and had a strong inhibitory effect on β-hexosaminidase released from RBL-2H3 cells after degranulation. Moreover, they showed significant down-regulation effects on the release of histamine, tumor necrosis factor-α and interleukin-4 from RBL-2H3 cell after degranulation, suggesting their potential as anti-allergic probiotics. The four strains were identified by 16S rDNA sequencing as two strains of Lactobacillus plantarum, one strain of L. fermentum and one strain of L. paracasei.

Key words: lactic acid bacteria; RBL-2H3 cells; probiotic properties; degranulation; anti-allergic activity

CLC Number:

TS201.3

Ma Ding, Qin Shuangxia, Hao Huichao, Deng Fangming, Zhao Lingyan. Screening and Identification of Probiotics with in Vitro Anti-Allergic Activity Based on the Degranulation Inhibition of RBL-2H3 Cells[J]. FOOD SCIENCE, 2024, 45(6): 55-63.

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Screening and Identification of Probiotics with in Vitro Anti-Allergic Activity Based on the Degranulation Inhibition of RBL-2H3 Cells

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