Abstract: With increasing consumer demands for high-quality yogurt products, the issue of post-acidification caused by the continuous acid production of starter cultures has become a critical bottleneck in industrial development. Traditional research approaches have struggled to systematically elucidate its underlying molecular mechanisms, whereas multi-omics technologies provide powerful tools for comprehensive analysis from genes to metabolites. Based on recent advances in the application of multi-omics technologies to the study of yogurt post-acidification, this review comprehensively​ summarizes the differentially expressed genes, functional proteins, and key metabolites identified through transcriptomic, proteomic, and metabolomic analyses. These omics data reveal dynamic changes in important biological processes during post-acidification, including acid tolerance, carbon metabolism reprogramming, and cellular homeostasis maintenance. By integrating multi-omics results, we construct a gene-protein-metabolite regulatory network to clarify the molecular basis underlying post-acidification. Integrated multi-omics analysis not only deepens our understanding of the mechanisms of post-acidification but also provides a new theoretical foundation for targeted regulation of starter culture metabolic activity and precise control of excessive acidification. These findings offer important guidance for the development of high-quality yogurt products.

Key words: yogurt; post-acidification; transcriptomics; metabolomics; proteomics; molecular regulation