FOOD SCIENCE ›› 1993, Vol. 14 ›› Issue (8): 61-63.

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A Method for the Determination of Vitamin C in Strawberry by Reversed -Phase High Performance Liquid Chromatography

 WANG  Wei-Dong   

  • Online:1993-08-15 Published:2011-12-30

Abstract:  A method for the determination of vitamin c in strawberry by reversed-phase high performance liquid chromatography has been reported in this paper. Vitamin c was separated on a Zorbax ODS (5μm) reversed-phase column using a solution of 0. 025 mol/L KH2PO4: methanol = 70:30 as the mobile phase and detected by ultraviolet spec-trophotometric detector at 254 nm. Good separation of Vitamin c was achieved in 2. 5 min . the range of detection is 0 -25 mg/L. The method was used for the analysis of actual samples and good results were obtained.

Key words:  , Reversed-phase High Performance Liquid Chromatography Strawberry Vitamin C0;