Abstract: This paper was on the preparation of alkali -insoluble glucan by the discarded brewer’s yeast autolysed beforehand. The alkali extraction method and the enzyme-alkali extraction method were carried out. The effects of the amount of NaOH and protease added, the extraction time, the concentration of NaOH on extracting glucan were studied in detail. Quantitative and qualitative analyses were carried out on the final preducts, and the results shew that 9(1 - 3) -D-glucan was the major component of the preducts.

Key words: &beta, (1-3) -D-glucan mannan yeast autolyse brewer’s yeast；