Abstract: High-purity of fructo-oligosaccharides (FOS) was prepared after glucose was removed in normal FOS products by glucose oxidase (GOD) and catalase (CAT). The optimum temperature and pH of glucose oxidase were 45℃and 5.0. respectively. 86.92 % of FOS was prepared by G0D (50U/g total sugar) and CAT (725U/g total sugar) at 30℃. pH 5.0, 3O% of total sugar concentration for 16 h aerated incubation.

Key words:  , Fructo-oligosaccharides Fructosyltransferase Glucose oxidase catalase；