Immobilization of Glucose Oxidase on the silk

Abstract

Abstract: The conditions of the glucose oxidase (GOD) immobilized on the silk have been studied, The results showed that the optimal conditions of the reaction between Silk and p-β-sulfuric acid ester ethyl sulfon aniline (SESA) are pH = 10. 8 and SESA: Silk = 1. 9: 1 (w/w). The product P--aminobenzent sulfaniliyl ethyl Silk (ABSE --Silk)was diazotized and linked with GOD. The optimal pH for glucose linked to diao -compound is 7. 0. The link time is at last 12h. When the amount Of GOD is 70 -110u/ g (Silk), the activity of immobilized COD is 35 -45u/ g (Silk) and the recovery rate of the enzyme is above 40%. The immobilized GOD is fairly stability, its operational half life is about 21 d.

Key words: , Glucose Oxidase Immobilization Silk SESA；

CAO Guo-Min, SHENG Mei, LENG Yi-Xin, GAO Guang-Da. Immobilization of Glucose Oxidase on the silk[J]. FOOD SCIENCE, 1998, 19(4): 3-6.

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Immobilization of Glucose Oxidase on the silk

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